Culturomic and quantitative real‐time <scp>‐</scp> polymerase chain reaction analyses for early contamination of abutments with different surfaces: A randomized clinical trial
نویسندگان
چکیده
Background Rough and/or plasma-activated abutments seem to be able increase soft tissue adhesion and stability; however, limited evidence is available about bacterial contamination differences. Purpose The aim was investigate the oral microbiota on four dental with different surfaces by quantitative real-time polymerase chain reaction (qRT-PCR) culturomic approach. Methods Forty patients needing a single implant rehabilitation were involved in study. healing abutments, especially designed for study, divided into groups according surface topography (1. machined [MAC], 2. plasma of argon treated [plasmaMAC], 3. ultrathin threaded microsurface [UTM], 4. UTM [Plasma UTM]). Random assignment performed predefined randomization tables. All underwent surgical intervention contextual abutment positioning. After 2 months healing, sterile cotton swab used microbiological sampling culturomics, while paper points inserted sulcus qRT-PCR. Results At end 36 completed all procedures total (9 per group) analyzed. qRT-PCR retrieved data 23 species whereas culturomics revealed presence 74 bacteria, most them not routinely included cavity kits analysis or never found before microenvironment. No statistically significant differences emerged analyzing (p = 0.053). On contrary, higher specific counts detected plasma-treated compared untreated ones 0.021). Conclusions Abutments topographies treatments resulted contaminated similar flora. moderately rough associated greater ones. Conversely, more bacteria around abutments. Furthermore, reported suggested include new previously sought routine analyses microflora.
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ژورنال
عنوان ژورنال: Clinical Implant Dentistry and Related Research
سال: 2021
ISSN: ['1708-8208', '1523-0899']
DOI: https://doi.org/10.1111/cid.13028